Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 SG4044 (LMBP 670)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | SG4044 |
| Accession number: | LMBP 670 |
| Depositor: | Prof. E. Remaut1 2 1 Department for Molecular Biomedical Research, VIB, Ghent, Belgium 2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium It was obtained from Dr S. Gottesman3 3 Laboratory of Molecular Biology, NCI, NIH, Bethesda, USA |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Medium: | LB-Lennox |
| Selection marker: | / |
| Temperature: | 37°C |
| Genotype: | lon-100 Δ(gal-pgl)324 lac thi rpsL |
| Phenotype: | StrR Lon- non-mucoid |
| Properties: | - |
| Further information: | The Lon protease plays a major role in the proteolytic machinery of E. coli. Lon mutants are highly pleioptropic and produce mucoid colonies that are difficult to handle. The (gal-pgl) deletion eliminates mucoidy and restores normal colony morphology. Lon mutants may be useful in stabilizing heterologous proteins expressed in E. coli (see e.g. Remaut et al., 1983). |
| Original reference: | Gottesman et al., J. Bacteriol. 148 (1981), 265-273 [PMID: 7026534] |
| Related reference: | Remaut et al., Nucleic Acids Res. 11 (1983), 4677-4688 [PMID: 6308555] Gottesman et al., J. Bacteriol. 162 (1985), 1111-1119 [PMID: 3888955] |
| Restricted use: | - BCCM MTA |
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Refer in your Materials and Methods: |
Escherichia coli K12 SG4044 (LMBP 670) is available at BCCM/GeneCorner. It was deposited by Prof. E. Remaut and was published in Gottesman et al., 1981. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.